Submitted to: International Plant Protection Congress
Publication Type: Abstract Only
Publication Acceptance Date: 1/15/2004
Publication Date: 5/1/2004
Citation: Holbrook Jr, C.C., Guo, B., Luo, M., Ozias-Akins, P. 2004. Use of molecular genetics to develop disease resistant peanut cultivars. Proc. 15th International Plant Protection Congress. p. 64. Interpretive Summary: not required.
Technical Abstract: Cultivated peanut (Arachis hypogaea L.) exhibits considerable variability for morphological and for resistance to diseases. Progress has been made in the development of disease resistant peanut germplasm using classical plant breeding techniques. The rate of progress could be accelerated with the use of molecular genetic technology, however, previous research with molecular markers has detected little variation at the DNA level in the cultivated peanut species. Additional research on marker development and gene discovery is needed. The development new technology using EST (expressed sequence tag) and EST-derived SSR (simple sequence repeat) should enable researchers to finally measure and exploit variation at the DNA level in cultivated peanut species. We have generated about 2000 EST's from two cDNA libraries of peanut, One library was developed using leaf tissue of C34-24, a lines with resistance to leaf and tomato spotted wilt virus (TSWV). The other library was developed using immature pod tissue of A13, a line with resistance to drought stress and preharvest aflatoxin contamination. This was the first report of EST development and frmnctional genomic research in peanut. Our research is now focused on screening these EST's to identify SSR markers. These markers will then be examined to identify those which are associated with resistance to biotic and abiotic stresses. These research efforts should greatly enhance marker assisted plant breeding and functional genomic research in peanut.