Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 2/1/2004
Publication Date: 3/1/2004
Citation: Chiemsombat, P., Thongsa, S., Phetsoongnern, P., Seetou, W., Adkins, S. T. 2004. Virus Diseases of Ornamental Hibiscus. Meeting Abstract.
Technical Abstract: The diagnosis of virus diseases of ornamental hibiscus or shoe flower, Hibiscus rosa-sinensis and H. hybrid, was conducted using tissue blot immunoassay techniques. Reverse transcription-polymerase chain reaction was used to amplify the viral coat protein (CP) gene from infected hibiscus and its nucleotide sequence was analyzed. The results showed that both hibiscus species were either singly or doubly infected by Hibiscus chlorotic ringspot carmovirus (HCRSV) and Hibiscus tobamovirus (HV). For H.rosa-sinensis, HCRSV and HV were detected in 118 and 64 plants from the total of 140 plants surveyed (84 and 46%, respectively. Among these, 52 plants (37%) had mixed infections. For H. hybrids, 50 of 72 plants (69%) surveyed were found to be infected by HV. Amplification of the HV CP gene from H.hybrids yielded a DNA fragment of 477 nucleotides, encoding a 158 amino acid residue (17,550 daltons) polypeptide characteristic of the HV CP. The Thai HV CP resembled that of Florida hibiscus virus reported in H. rosa-sinensis in the U.S.A. By mechanical inoculation, this Thai isolate of HV could infect and cause severe mosaic on leaves of some malvaceous plants such as okra (Abelmoschus esculentus), red sorrel (Hibiscus sabdariffa) and cotton (Gossypium hirsutum), which are economically important crops in Thailand.