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United States Department of Agriculture

Agricultural Research Service

Title: RICE PI-TA GENE CONFERS RESISTANCE TO THE MAJOR PATHOTYPES OF THE RICE BLAST FUNGUS IN THE U.S.)

Author
item Jia, Yulin
item Wang, Zhonghua
item Singh, Pratibha
item Redus, Marc
item Fjellstrom, Robert
item Johnson, Virginia
item Correll, James
item Lee, Fleet
item Rutger, J

Submitted to: Annual International Plant & Animal Genome Conference
Publication Type: Abstract only
Publication Acceptance Date: 12/31/2003
Publication Date: 1/10/2004
Citation: Jia, Y., Wang, Z., Singh, P., Redus, M., Fjellstrom, R.G., Johnson, V., Correll, J., Lee, F., Rutger, J.N. 2004. Rice Pi-ta gene confers resistance to the major pathotypes of the rice blast fungus in the U.S. [abstract]. Annual International Plant & Animal Genome Conference. Abstract P293, p. 145.

Interpretive Summary:

Technical Abstract: The Pi-ta gene in rice prevents the infection of Magnaporthe grisea isolates containing the avirulence AVR-Pita gene. Pi-ta encodes a putative cytoplasmic receptor that appears to bind to a predicted processed AVR-Pita to elicit a defense response. The presence of the Pi-ta gene was detected in 13 rice cultivars that reportedly contain Pi-ta. All Pi-ta containing cultivars were resistant to both major pathotypes IB-49 and IC-17 of M. grisea. The inheritance of resistance to IC-17 was further investigated using a marker for the resistant Pi-ta allele in an F2 population of 1345 progeny of the cross with a Pi-ta containing rice cultivar. Resistance to IC-17 was conferred by a single dominant gene, and Pi-ta was not detected in susceptible individuals. Another F2 population of 377 individuals of a reciprocal cross was used to verify the conclusion that resistance to IC-17 was conferred by a single dominant gene. In this cross, individuals resistant to IC-17 were also resistant to IB-49. The presence of Pi-ta and resistant to IB-49 was also correlated with additional crosses involving another Pi-ta containing rice culitvar. A pair of primers that specifically amplifies a susceptible pi-ta allele was developed to verify the absence of the dominant Pi-ta gene. These data suggest that Pi-ta is responsible for resistance to IB-49 and IC-17. The correlation of Pi-ta with resistance to both M. grisea pathotypes suggesting they contain functional AVR-Pita genes. Progress on developing molecular markers to facilitate the incorporation of Pi-ta into improved rice germplasm will be described.

Last Modified: 8/24/2016
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