Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 12/10/2003
Publication Date: 5/1/2004
Citation: Vuong, T., Zou, J., Hartman, G.L., Clough, S.J. 2004. Microarray analysis of defense against sclerotinia stem rot in soybean [abstract]. Plant and Animal Genome Conference.
Technical Abstract: Sclerotinia stem rot (Sclerotinia sclerotiorum), also known as white mold, is a major disease of soybean in the north-central region of the United States. Partial resistance to this pathogen has been reported, yet understanding of the molecular basis of resistance is limited. cDNA microarray technology provides an efficient tool to facilitate searching for genes involved in quantitative traits, such as in defense against Sclerotinia stem rot. The goal of our study was to analyze the profiles of differentially expressed genes in stem tissues of soybean seedlings inoculated with the fungal pathogen at several time points. Two soybean genotypes, Williams 82 (S) and PI194639 (R), were grown hydroponically in a growth chamber under controlled conditions of light intensity and temperature. Cut stem inoculation was performed when soybeans were 2 weeks old. Total RNA samples from stems collected at 0, 3, 6, 18, and 48 h after post inoculation were fluorescently labeled with Cy3 or Cy5 dyes and hybridized onto soybean microarrays containing over 9,000 gene representatives. Analysis of variance (ANOVA) assays were performed to identify genes showing significant differential expression. Profiles of PI194639 were compared to that of Williams 82 to identify candidate defense-related genes.