Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 10/1/2003
Publication Date: 1/10/2004
Citation: Zou, J., Rodriguez-Zas, S., Tuteja, J., Gonzales, D.O., Vodkin, L.O., Clough, S.J. 2004. Gene expression profiling of soybean response during compatible and incompatible interactions with the bacterial pathogen pseudomonas syringae [abstract]. Plant and Animal Genome Conference. p.273.
Technical Abstract: The interaction between a pathogen and a plant host is often classified as being either compatible (disease) or incompatible (defense). Defeating the pathogen depends on the ability of the host to respond rapidly and aggressively through the proper activation and repression of numerous genes. To detail the genetic responses that occur under both interactions, we performed large-scale mRNA expression profiling using cDNA microarrays representing over 27,000 soybean expression sequence tags. Soybean leaves (Williams 82) were infiltrated with Pseudomonas syringae pv. glycinea with or without avrB, or 10 mM MgCl2 (as control). RNAs were sampled at 0, 2, 8, and 24 hour post inoculation (hpi). By replicating our experiments with a loop design and using a statistical analysis (ANOVA), significant changes in transcription level were revealed. For example, during the incompatible interaction, over 14% of the 27,000 genes monitored showed significant expression level changes in at least one of the time points. In addition, many genes showed significant differences between the compatible and incompatible interactions at both the 8 and 24 hpi time points. We further classified significant genes according to Gene Ontology (GO) terms and as members of selected defense-related pathways, allowing for the observation of regulation patterns. In addition, TaqMan analysis was performed on members of the CHS gene family to distinguish which members of this family are specifically responding to the pathogens. These global gene expression results allow for cross comparison studies to other studies involving plant-pathogen interactions of soybean as well as those of other plant species.