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ARS Home » Midwest Area » Columbus, Ohio » Soil Drainage Research » Research » Publications at this Location » Publication #155213
Title: INSIGHTS INTO FLOODING TOLERANCE OF SAG12:IPT ARABIDOPSIS PLANTS USING TRANSCRIPTOME ANALYSES

Author
item Vantoai, Tara
item LIU, FENGLONG - TIGR
item MOY, LINDA - TIGR
item HUYNH, LE NGUYEN - OHIO STATE UNIVERSITY
item Banowetz, Gary
item QUACKENBUSH, JOHN - TIGR

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 10/3/2003
Publication Date: 1/1/2004
Citation: Vantoai, T.T., Liu, F., Moy, L., Huynh, L., Banowetz, G.M., Quackenbush, J. 2004. Insights into flooding tolerance of sag12:ipt arabidopsis plants using transcriptome analyses. Plant and Animal Genome Abstracts. p.W4.

Interpretive Summary:

Technical Abstract: Transgenic Arabidopsis plants containing the SAG12:ipt autoregulated cytokinin production capability remain greener and produce more biomass and seeds than wild-type (WT) plants under flooding stress. Flooding induces a large increase in ipt transcript abundance in SAG12:ipt (IPT) plants which is accompanied by increased cytokinin accumulation. To profile changes in gene expression that contribute to flooding tolerance, whole genome DNA microarray analysis representing the more than 27,000 genes encoded in the Arabidopsis nuclear, chloroplast and mitochondrial genomes was conducted at specific times during a 5-day period of non-flooded control, waterlogging and complete submergence. Average linkage hierarchical clustering using Euclidean distance metric revealed distinct patterns of gene expression in the three treatments. Under flooding stresses, genes of photosynthesis and energy utilization pathways expressed 2- to 4-fold more in the tolerant IPT plants than in WT plants suggesting that flooding tolerance mechanisms involve both energy production and utilization. A number of transcription regulators and genes of signaling pathways were induced specifically in flooded IPT plants. Comprehensive analysis of transcriptional changes associated with cytokinin-induced flooding stress tolerance will be reported.