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Title: METABOLIC PROFILING OF THE SHORT-TERM ARABIDOPSIS THALIANA RESPONSE TO INCREASED CO2 LEVELS USING GAS CHROMATOGRAPHY-MASS SPECTROMETRY

Author
item KANANI, H - UNIVERSITY OF MARYLAND
item KLAPA, M - UNIVERSITY OF MARYLAND
item Vantoai, Tara
item MOY, L - INSTITUTE FOR GENOMIC RES
item LINFORD, L - INSTITUTE FOR GENOMIC RES
item HASSEMAN, J - INSTITUTE FOR GENOMIC RES
item QUACKENBUSH, J - INSTITUTE FOR GENOMIC RES

Submitted to: Mid Atlantic Plant Molecular Biology Society Conference
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2003
Publication Date: 8/7/2003
Citation: KANANI, H., KLAPA, M.I., VANTOAI, T.T., MOY, L., LINFORD, L., HASSEMAN, J., QUACKENBUSH, J. 2003. METABOLIC PROFILING OF THE SHORT-TERM ARABIDOPSIS THALIANA RESPONSE TO INCREASED CO2 LEVELS USING GAS CHROMATOGRAPHY-MASS SPECTROMETRY. Mid Atlantic Plant Molecular Biology Society Conference. p. 28.

Interpretive Summary:

Technical Abstract: The reaction of plant physiology to elevated CO2 concentrations in the plant growth environment has been the focus of numerous studies, in an effort to understand the effect of potential increase of CO2 level in the environment on the growth and the product yield of plants. However, the majority of the previous studies monitored the long-term response of the plants after long exposure to high CO2 levels. In this study, we measured the short-term physiological response of A. thaliana (Columbia strain) liquid cultures to increased CO2 concentration in their growth environment at the metabolic level. Specifically, the experiment involved the growth of two sets of plants for 12 days in Gamborg media under constant light and 23ºC. On the 13th day, the two sets of plant liquid cultures were fed air of ambient composition ("control set") and of 1% CO2 ("perturbed set"), respectively, which were harvested at different time points during the day. Gas Chromatography - Mass Spectrometry was used to measure the average (over the entire plant) metabolic plant profiles, in an effort to identify the characteristic metabolic fingerprint underlying the reaction of the A. thaliana physiology to the imposed CO2 stress. Metabolic profiling refers to the qualitative and quantitative detection of a variety of low molecular weight intracellular metabolites, including organic acids, amino acids, sugars, sugar alcohols, amines and saccharides, obtained after the break-up of the cellular macromolecules. In this study, we used the methanol extraction protocol described by Roessner et.al. [1], using ribitol as the internal standard. Taking into consideration that enzymatic activity affects and is affected by the concentration of intracellular metabolites, the metabolic profiles can provide an extensive picture of the metabolic state of a complex organism. We present the obtained metabolic profiles in the context of the known A. thaliana metabolic network structure and regulation to derive conclusions about the metabolic response of the plant to the external stress. We plan to combine the final conclusions from this analysis with the results from the transcriptional profiling analysis of the same plant liquid cultures (see Dutta et. al.) to identify similarities and differences between the genomic and metabolic short-term reaction of the plant to elevated levels of CO2 in their growth environment.