Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/14/2001
Publication Date: 6/22/2002
Citation: RAJESH, P.N., TULLU, A., GIL, J., GUPTA, V.S., RANJEKAR, P.K., MUEHLBAUER, F.J. IDENTIFICATION OF AN STMS MARKER FOR THE DOUBLE-PODDING GENE IN CHICKPEA. JOURNAL OF THEORETICAL AND APPLIED GENETICS. 2002. v. 105. p. 604-607. Interpretive Summary: Double podding, the ability of the plant to form two pods rather than one pod per reproductive site is considered a yield-enhancing trait for chickpea breeding. In this study we determine the location of the gene for double podding in the chickpea genome and through the use of DNA techniques, we identified markers closely linked to the gene. With this information, it is now possible to determine the genetic make up of chickpea breeding lines and facilitate selection for this important trait. The information will enable the selection of double podding germplasm and will benefit producers through the development of higher yielding cultivars.
Technical Abstract: Chickpea, a self-pollinating diploid annual with 2n=2x-16 chromosomes, is an important food legume crop throughout the world and especially in developing countries. A gene that confers double-podding, with the symbol ¿s¿, is considered important for breeding higher yielding cultivars. Positive effects of ¿s¿ on yield stability were found by comparing single- and double-podded near-isogenic lines (NILs) derived from a cross of CA-2156 (single-podded) with JG-62 (double-podded). Considering the significant effects on seed yield, the NILs were used to identify molecular markers closely linked to ¿s¿. Sequence tagged microsatellite site (STMS) markers developed for chickpea, random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers were used to analyze the NILs. Out of 400 RAPD, 100 STMS and 100 ISSR markers, one STMS marker (TA-80) was polymorphic and was used to evaluate a recombinant inbred line population developed from the cross of Surutato-77 (single-podded) x JG-62 (double-podded) for co-segregation of the locus with ¿s¿. Our results indicated that the marker and ¿s¿ were 4.84 cM apart. This marker may be used by breeders for marker-assisted selection (MAS) to monitor the incorporation of the double-podding gene into improved germplasm.