Submitted to: Fungal Genetics Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 1/16/2003
Publication Date: 2/28/2003
Citation: WEILAND, J.J. TRACKING DNA POLYMORPHISMS IN FIELD POPULATIONS OF APHANOMYCES COCHLIOIDES. FUNGAL GENETICS CONFERENCE. 2003. P. 143. ABSTRACT NO. 445.
Technical Abstract: Root and seedling disease caused by Aphanomyces cochlioides are serious impediments to sugarbeet production in wet growing regions, yet information on the genetics and the inheritance of virulence in this organism is lacking. No race structure for A. cochlioides has been reported and several studies have revealed limited genetic diversity in this oomycete using DNA-based technologies. In the present study, application of random amplified polymorphic DNA (RAPD) analysis to single zoospore isolates obtained from sugarbeet fields in the U.S. identified 2 polymorphisms that assorted randomly within local populations; some field populations harbored only one polymorphic type. The data indicate that these polymorphisms are found in A. cochlioides isolates ranging from the northern Red River Valley of the U.S. to the historic regions of sugarbeet production in Texas. Implications of this result in the development of novel virulence and fungicide resistance in A. cochlioides are discussed.