|LOMINADZE, DAVID - UNIV OF LOUISVILLE
|SCHUSCHKE, DALE - UNIV OF LOUISVILLE
Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 12/2/2002
Publication Date: 3/14/2003
Citation: Lominadze, D., Saari, J.T., Schuschke, D.A. 2003. Neutrophil migration under agarose is altered by copper deficiency [abstract]. The Federation of American Societies for Experimental Biology Journal. 17:A1128.
Technical Abstract: Dietary copper deficiency alters neutrophil sequestration and margination within the pulmonary microcirculation in rats. In the current study, the role of copper in CD1 lb/CD18-dependent an -independent migration of neutrophils under agarose was studied. Male, weanling Sprague-Dawley rats were fed purified diets that were either copper-adequate (CuA, 6.3 ug Cu/g diet) or copper-deficient (CuD, 0.3 ug Cu/g diet) for 4 weeks. Neutrophils from CuA and CuD rats were isolated using a continuous density gradient created in Polymorphprep. Migration of neutrophils under agarose was studied in response to fMLP (10**-9 mM), which activate CD1 lb/CD18-dependent and -independent pathways, respectively. Neutrophil migration was analyzed by direct observation of the Giemsa stained samples under the microscope. fMLP-induced neutrophil migration was significantly slower in CuD compared to the CuA group; however, IL-8-induced neutrophil migration was significantly faster in the CuD group than in the CuA group. These results suggest a copper deficiency-induced depression of the CD1 lb/CD18-mediated neutrophil migration pathway with a coincident enhancement of CD1 lb/CD18-independent migration. These alterations of the copper-deficient neutrophils may contribute to the increased margination of neutrophils seen in the copper-deficient rat lung. Supported by NIH DK55030.