|Buckler, Edward - Ed|
Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 3/14/2002
Publication Date: 3/14/2002
Citation: LIU, D., FROST, J., SZALMA, S., ANDALUZ, S., MUSKET, T., BUCKLER IV, E.S., MCMULLEN, M.D., DAVIS, G. ASSOCIATIONS BETWEEN GLOSSY15 POLYMORPHISMS AND VARIATION IN JUVENILE LEAF NUMBER. MAIZE GENETICS CONFERENCE. 2002. ABSTRACT. P. 118.
Technical Abstract: Maize lines with fewer juvenile leaves are more resistant to fall armyworm and southwestern corn borer infestation during the whorl stage. This increased Lepidopteran resistance is likely due to differences in the chemical constituents of the wax layer of juvenile verses adult leaves. The goal of this project is to identify one or more single nucleotide polymorphisms (SNP) associated with reduced juvenile leaf number than can be used to screen for more insect resistant corn lines or in marker-assisted selection. Since number of juvenile leaves varies between lines of maize, 100 maize inbred lines (52 U.S. lines, 7 European and Canadian lines, and 41 Tropical/semitropical lines) were used to identify the lines with the lowest and highest number of juvenile leaves. One hundred lines of maize were grown in two replications and data were collected for the last juvenile, the first fully adult, and total number of leaves. Number of transition and fully adult leaves were then calculated from collected data set. Juvenile leaf number varies between 3.00 to 7.36 leaves per plant with a mean of 4.90, while the adult leaf number ranged from 4.39 to 23.25 leaves per plant with a mean of 11.54. There was no relationship between the origin of lines and juvenile leaf number. The maize Glossy15 (Gl15) locus is a loss of function mutation that causes abbreviated expression of juvenile leaves. Fifteen primer pairs for PCR amplification were selected form the GenBank sequence of Gl15. Of the 15 initial primer pairs, two amplified large robust single fragments. These were selected for use in sequencing of the various inbred alleles. Association analysis was first performed on a subset of 32 maize inbred lines to evaluate potential associations between Gl15 sequence polymorphisms and variation in the number of juvenile or adult leaves.