Submitted to: Crop Science
Publication Type: Peer reviewed journal
Publication Acceptance Date: 3/27/2003
Publication Date: 8/1/2003
Citation: Wu, J., Jenkins, J.N., Zhu, J., McCarty, J.C., Watson, C.E. 2003. Comparisons of quantitative trait locus mapping properties between two methods of recombinant inbred line development. Euphytica. 132:159-166. Interpretive Summary: Recombinant inbred populations of cotton are very useful for determining the associations among quantitative trait loci for important genetic traits and molecular markers. The conventional way to develop these populations is by single seed descent. This is very expensive in terms of effort and labor. We used an alternative way to develop these recombinant inbred populations by bulk self pollination of individual F2 family lines until the F5 or F6 generation and then developed self pollinated lines from individual plants in each family. We used Monte Carlo simulations with various population sizes, heritability of traits, and quantitative trait loci effects to compare the bulk method of development of recombinant inbred populations to the single seed descent method. The results showed that our bulked developed recombinant inbred populations could provide similar results to single seed descent populations if 75 or more populations were used with 2 lines developed in each population. Thus, bulked based population development for recombinant inbred populations provides a more economical way to develop useful recombinant inbred populations in cotton.
Technical Abstract: The theoretical comparisons for QTL mapping properties were conducted between bulk-based recombinant inbred (RI) populations and single seed descent (SSD) RI populations by Monte Carlo simulations based on various population sizes, heritabilities, and QTL effects. The comparisons included estimation of QTL effects and QTL positions between two types of RI populations. The simulations showed that bulk-based RI populations consisting of 75 families (or more) with two lines per family could provide similar mapping results to the same size SSD RI populations. The study suggested that the bulk-based-method could also be used to develop large numbers of RI lines for linkage mapping and QTL mapping while development of a large SSD RI population is practically difficult.