Submitted to: Arkansas Agricultural Experiment Station Research Series
Publication Type: Experiment Station
Publication Acceptance Date: 8/1/2002
Publication Date: 8/10/2002
Citation: Eizenga, G.C., Lee, F.N., Jia, Y. 2002. USE OF DNA MARKERS TO IDENTIFY BLASTS RESISTANCE GENES IN THE WILD RELATIVES OF RICE (ORYZA SP.). Arkansas Agricultural Experiment Station Research Series. Exp. Stn. Res. Ser. 495. p.19-23. Interpretive Summary: Interpretive summary not required.
Technical Abstract: Blast (Pyricularia grisea Cav.) is the major fungal disease affecting rice (Oryza sativa L.) in the USA. Wild species often have served as sources of new and unique disease resistance genes for crop plants. Research objectives were to 1) identify polymorphic microsatellite (DNA) markers, especially markers located in blast resistant regions in order to follow the introgression of new blast resistance genes from Oryza sp. into cultivated rice, 2) screen for the known blast resistance gene, Pi-ta, and 3) determine if the Oryza spp. accessions were resistant to the US blast races IB-1, IB-33, IB-49, IB-54, IC-17, IE-1K, IG-1 and IH-1. To date, 33 of the 58 microsatellite markers screened were polymorphic for at least one cross with cultivated rice and 16 were located in the region of blast resistance genes. Blast resistance gene, Pi-ta, was present in some of the O. nivara accessions and the O. rufipogon accession. O. nivara and O. rufipogon accessions had resistance to the US blast races. Oryza spp. are being crossed with blast susceptible M-201 to further identify new blast resistance genes.