Submitted to: Plant Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 10/11/2001
Publication Date: 1/12/2002
Citation: Levi, A., Thomas, C.E., Joobeur, T., Zhang, X., Davis, A. 2002. Genetic linkage map for watermelon based on a testcross population: (citrullus lanatus var. citroides x c. lanatus var. lanatus) x c. colocynthis. Plant Genome Conference Proceedings. (p #673)
Technical Abstract: A genetic linkage map was constructed for watermelon using a testcross population [Plant Accession Griffin 14113 (C. lanatus var. citroides) x New Hampshire Midget (NHM; Citrullus lanatus var. lanatus)] x U.S. Plant Introduction (PI) 386015 (C. colocynthis). The map contains 141 randomly amplified polymorphic DNA (RAPD) markers produced by 78 primers, 27 inter- simple sequence repeat (ISSR) markers produced by 17 primers, and a sequence characterized amplified region (SCAR) marker that was previously reported as linked (1.6 cM) to race 1 Fusarium wilt (incited by Fusarium oxysporum Schlechtend.:Fr. f. sp. niveum [E.F.Sm.] W.C. Synder & H.N. Hans) resistance in watermelon. The map consists of 25 linkage groups. Among them a large linkage group containing 22 markers covers a mapping distance of 225.6 cM and six large linkage groups, each with 10 to 20 markers cover a mapping distance of 68.8 to 110.8 cM. There are five additional linkage groups, each consisting of 3 to 7 markers that cover a mapping distance of 36.5 to 57.2 cM. The thirteen remaining linkage groups are small, each consisting of 2 to 11 markers covering a mapping distance of 3.5-29.9 cM. The entire map covers a total distance of 1166.2 cM with an average distance of 8.1 cM between two markers. The map is useful for further development of markers linked to disease resistance and watermelon fruit qualities.