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ARS Home » Pacific West Area » Aberdeen, Idaho » Small Grains and Potato Germplasm Research » Research » Research Project #442383

Research Project: Correlation Between Beta-glucan Hydrolase Activity in Seedling and Malt Beta-glucan Content

Location: Small Grains and Potato Germplasm Research

Project Number: 2050-21000-038-012-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2022
End Date: Aug 31, 2024

Beta-glucan content in barley malt is the number quality trait to determine whether the barley variety will be accepted by malting industry. Genetic background and environmental effect on the trait are very complicated. This proposed project is to investigate whether the beta-glucan hydrolase activities are responsible for the malt content.

ARS barley breeding program has evaluated some elite barley lines indifferent growing environments for their malt beta-glucan contents. Proteomics analysis of four genotype samples from five growing environments showed that some beta-glucan hydrolase enzymes are uniquely expressed with one genotype in the low beta-glucan content growing environment. University of Idaho collaborator will use their expertise and facilities to measure the beta-glucan hydrolase activity for all the barley seed samples collected from all five environments. Then to correlate the enzyme activity to the malt beta-glucan content.