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ARS Home » Southeast Area » Fort Pierce, Florida » U.S. Horticultural Research Laboratory » Subtropical Plant Pathology Research » Research » Research Project #445333

Research Project: Control Citrus Huanglongbing by Using The Citrus Isolate of Nectarine Marafivirus M and its Expression System

Location: Subtropical Plant Pathology Research

Project Number: 6034-22000-045-025-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Mar 1, 2024
End Date: Feb 28, 2025

1. Characterizing the NMV-M citrus isolate, including its host range and transmission. 2. Using NMV-infected citrus to prevent or suppress HLB in greenhouse and field trials.

Objective 1. Our preliminary data indicates that the virus can be detected in ACP at relatively high titers. We will focus our transmission study in part on major citrus-feeding insects, such as ACP and leafhopper, and determine the virus’ host range by graft and dodder transmission. We will use electron microscopy to illustrate how both the virus and Las bacteria co-exist in a phloem cell and how the virus attenuates the phloem cell destruction caused by Las. Objective 2. The systemic distribution of NMV-M/CFL will be evaluated among different varieties. We will expand the screening to all important commercial citrus varieties to provide more virus and host combinations for field trials. We will further conduct a small-scale field trial of new planting or grafting NMV-M to HLB-affected plants at the USHRL Picos farm to evaluate the prevention and/or revitalization of HLB-affected plants.