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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Meat Safety and Quality » Research » Research Project #444055
Research Project: Develop/Validate “Next Generation” Methods (e.g., Gene-based Approach) for Detecting Foodborne Pathogens of Concern

Location: Meat Safety and Quality

Project Number: 3040-42000-020-015-A
Project Type: Cooperative Agreement

Start Date: May 1, 2023
End Date: Dec 14, 2023

Objective:
To use CRISPR-SeroSeq, or Deep Serotyping, technology developed by the Cooperator, to characterize the diversity of Salmonella present in FSIS enrichment samples (n=1260) that contain multiple serotypes. These data will lay the groundwork for improving Salmonella detection methods when multiple serotypes are present, and will be used to help identify Salmonella serotype bias in enrichment culture media.

Approach:
CRISPR-SeroSeq analysis will be used to identify the diversity of Salmonella serotypes present in 1260 Salmonella positive enrichments from beef, pork, chicken and turkey samples. These data will help define serotype diversity and relative abundance in various meat enrichments and will help determine what tools or specific enrichment methods are needed to identify Salmonella of greater concern for human health, when multiple serotypes are present in a sample.