Location: Small Grains and Potato Germplasm Research
Project Number: 2050-21000-038-012-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 1, 2022
End Date: Aug 31, 2024
Objective:
Beta-glucan content in barley malt is the number quality trait to determine whether the barley variety will be accepted by malting industry. Genetic background and environmental effect on the trait are very complicated. This proposed project is to investigate whether the beta-glucan hydrolase activities are responsible for the malt content.
Approach:
ARS barley breeding program has evaluated some elite barley lines indifferent growing environments for their malt beta-glucan contents. Proteomics analysis of four genotype samples from five growing environments showed that some beta-glucan hydrolase enzymes are uniquely expressed with one genotype in the low beta-glucan content growing environment. University of Idaho collaborator will use their expertise and facilities to measure the beta-glucan hydrolase activity for all the barley seed samples collected from all five environments. Then to correlate the enzyme activity to the malt beta-glucan content.
