Location: Sunflower and Plant Biology Research
Project Number: 3060-21220-034-021-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Jul 1, 2022
End Date: Dec 31, 2023
1. Use gene editing tools to enhance the endogenous oxalic acid catabolism pathway in Brassica napus to more efficiently metabolize oxalic acid and enhance resistance to Sclerotinia sclerotiorum; and 2. Develop Brassica napus germplasm that has decreased sensitivity to oxalic acid and enhanced resistance to S. sclerotiorum.
Utilizing molecular genetic tools including gene cloning, heterologous and endogenous expression of plant genes, CRISPR-based technology for non-transgenic mutagenesis, transcriptomic analysis of gene expression dynamics and host infection studies, we will identify genes and pathways required for host metabolism and response to pathogen-produced oxalic acid. These genes will be manipulated first transgenically in Arabidopsis thaliana and then cis-genically, through CRISPR technology, in Brassica napus to enhance tolerance to oxalic acid and provide resistance to Sclerotinia sclerotiorum.