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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Mycotoxin Prevention and Applied Microbiology Research » Research » Research Project #441379

Research Project: Explore Novel RNA Strategies Using Fungal Endophyte to Control Mycotoxin Contamination of Corn

Location: Mycotoxin Prevention and Applied Microbiology Research

Project Number: 5010-11420-001-006-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Oct 1, 2021
End Date: Sep 30, 2024

Objective: Develop an endophyte-based delivery system for double-stranded RNA (dsRNA) to control Fusarium mycotoxin production and mitigate associated corn crop losses.

We propose to develop an innovative method to deliver dsRNAs efficiently and continuously to maintain the RNAi effect in planta using an endophyte delivery system. We will utilize a corn endophyte to continuously produce and supply RNAi signals to combat Fusarium pathogens and mycotoxin contamination. We will introduce dsRNA constructs already developed by ARS into a corn endophyte, inoculate variable corn varieties with the transgenic endophyte, and then determine the RNAi silencing efficacy targeting these critical mycotoxin biosynthesis genes of Fusarium graminearum and Fusarium verticillioides.