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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Genetic Improvement for Fruits & Vegetables Laboratory » Research » Research Project #440645

Research Project: Genotyping and Phenotyping of Cranberry for Genome Wide Association Mapping

Location: Genetic Improvement for Fruits & Vegetables Laboratory

Project Number: 8042-21000-279-014-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2021
End Date: Aug 30, 2022

Objective:
We are breeding superior cranberry varieties using modern techniques. This specifically requires rapid and accurate genotyping (in other words, techniques to determine differences in the DNA) as well as phenotyping (measurement of important traits). Together, these data allow us to speed the breeding process. The ojectives of the agreement are: 1) Genotype cranberry germplasm using capture-seq; 2) Collect cranberry phenotype data using various techniques and approaches; 3) Utilize the resulting data to perform GWAS (genome wide association mapping); and 4) Develop markers for desired traits based on GWAS results.

Approach:
1. Cranberry leaf samples will be collected from the Rutgers University germplasm collection (576 samples). 2. Genotyping will be performed using next generation sequencing and specifically, capture-seq, using 15,000 custom probes. 3. Sequencing data will be compiled and genome wide association analysis (GWAS) will be performed. 4. Rutgers and ARS will collect phenotype data.