Location: Sunflower and Plant Biology Research
Project Number: 3060-21000-047-008-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 1, 2020
End Date: Aug 31, 2024
Objective 1: Whole genome resequencing of a mapping population segregating for hull hardness, hull thickness, and oil content. Objective 2: Measurement of hull hardness independent of hull thickness in the mapping population, and QTL mapping of the trait.
To better understand hull thickness, its correlation with insect resistance and oil content, and enhance it for breeders and producers, we propose two objectives. The first is to resequence a mapping population of sunflower that is segregating for hull hardness, hull thickness, and oil content. Tissue will be provided by USDA-ARS in Fargo, for extraction and skim sequencing at CU or an external service lab. Variant calling and quality control will be conducted. The second objective is for a summer student to study the mapping population in the field, collect seed samples as the develop in the plant, and subject them to hull thickness and hull strength measurements via a penetrometer. These data and oil content will then be combined with the variant calls from the first objective to conduct QTL mapping of the traits for subsequent publication.