Location: Poultry Research
Project Number: 6064-13000-013-007-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Jul 31, 2019
End Date: Jul 30, 2024
The objective of this research is to assess effects of Escherichia (E.) coli mitigation in commercial poultry production as affected by rearing practices, litter management practices, and differing environmental management strategies on production efficiency and resource use.
Three bio-aerosol samplers, an Andersen six-stage impactor, an all-glass impinger (AGI-30), and a dry air sampler (SASS) will be tested in this study. Operated at an airflow of 28.3 L min-1, The Andersen impactor separately collect airborne microorganisms > 7.1 µm, 4.7-7 µm, 3.3-4.7 µm, 2.1-3.3 µm, 1.1-2.1 µm, and 0.65-1.1 µm, respectively, in its six stages. Each stage contains a glass petri-dish with 27 mL MacConkey No. 3 agar that collects airborne particles on the agar surface though inertial impaction. Six liquid samples will be prepared by rinsing the collected particles off the agar surface at each stage using 2 mL buffered peptone water (BPW). The AGI-30 collects airborne microorganisms into 20 mL BPW with 0.005% silicone antifoam. The nominal airflow of AGI-30 is 12.5 L min-1. The SASS sampler collects airborne microorganisms on a high efficiency filter at a high airflow of 300 L min-1. After sampling, the SASS filter will be eluted in 10 mL BPW. Air samples of all samplers will be prepared in the liquid form. System setup and air sampling: Litter from three commercial broiler and three cage-free hen farms will be collected and stored in a container placed inside the biosecurity cabinet. The litter will be inoculated with non-pathogenic Escherichia (E.) coli. The amount of E. coli for inoculation will be adjusted to generate enough airborne E. coli for sampling. The airborne particles will be generated through raking the litter using an automated raking system. The raking simulates the foraging behavior of birds in commercial settings. Three bio-aerosol samplers will be placed side- by-side and perform air sampling simultaneously. The duration of air sampling will be 30 mins.