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ARS Home » Southeast Area » Mississippi State, Mississippi » Poultry Research » Research » Research Project #437202

Research Project: Performance and Physiological Characteristics of Poultry Vaccinated and/or Challenged with Avian Pathogenic Escherichia coli (APEC)

Location: Poultry Research

Project Number: 6064-13000-014-004-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Jul 31, 2019
End Date: Jul 30, 2024

Objective:
Apply in ovo-based vaccine delivery technologies for the delivery of live attenuated APEC vaccines.

Approach:
To develop and assess in ovo-based delivery methods for a live attenuated APEC vaccine (Poulvac™ E. coli), fertile eggs will be obtained from a commercial source and will incubated as recommended by the supplier. Treatments will include 2 vaccine delivery sites (amnion, air cell) and multiply dosages. Prior to injection at 18 days, eggs will be screened for viability, live embryos will be injected according to treatment, and eggs will be transferred to hatching baskets. Following hatch, all treatment-associated chicks will be quantified to assess hatchability. Chicks will be swabbed to assess the presence of the Poulvac™ E. coli which will be performed utilizing a vaccine specific Taqman PCR assay. A subset of randomly (10%) selected hatchlings from each treatment will be reared through 6 weeks of age in biological isolation chambers for assessment of livability and humoral immune response. Feed and water will be provided ad libitum and lighting and temperature will be as described by their source (e.g. Hy- Line Red Book, Hy-Line International 2014). Mortality will be recorded daily, and all mortality will be necropsied and sampled for the presence of the Poulvac™ E. coli vaccine. At 6 weeks of age, all birds will be weighed, swabbed (choanal cleft, esophagus, and cloaca) to assess the presence of the Poulvac™ E. coli and bled (wing vein) for ELISA (IgY) assays to assess the humoral response.