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ARS Home » Pacific West Area » Aberdeen, Idaho » Small Grains and Potato Germplasm Research » Research » Research Project #436926

Research Project: Screening Barley Germplasm to Discover Genes Conferring Durable Resistance to Barley Stripe Rust

Location: Small Grains and Potato Germplasm Research

Project Number: 2050-21000-034-27-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Jul 1, 2019
End Date: Jun 30, 2021

Objective:
Discover, characterize, develop, and deploy effective resistance against barley stripe rust (BSR) (incited by Puccinia striiformis f. sp. hordei) to protect U.S. barley production via the following activities by participating in a coordinated germplasm evaluation effort involving USDA-ARS Aberdeen and Pullman; Oregon State University, Corvallis; and University of California, Davis; and 2) Collaborate with participating scientists in the development and deployment of germplasm resistant to BSR.

Approach:
Coordinate germplasm assembly, testing protocols, and final data analysis and dissemination. Test germplasm, using a set of materials, phenotyping protocols, and experimental designs that are common to the three test locations (Davis, CA; Mt. Vernon, WA; and Corvallis, OR). Phenotyping will be done in single-row plots using procedures that are standard at each location. Susceptible spreader rows composed of susceptible varieties, and artificial inoculation (if warranted) will be used to facilitate infection. Minimum data: barley stripe rust severity at one assessment date, heading date, other diseases. Disease samples will be sent to the USDA-ARS Pullman lab for race identification. Several categories of germplasm will be evaluated: (A) PNW BSR screening trial: This nursery, including checks, will consist of ~ 40 entries composed of mixture of new and previously-tested entries from California, Idaho, Oregon, and Washington public breeding programs, resistant and susceptible checks, and a set of differentials. This trial will be grown at each location using a minimum of three replicates. (B) Novel germplasm/population screening: These nurseries, consisting of GWAS panels and/or biparental populations, will be used to identify new sources of resistance, elucidate the genetics of barley stripe rust resistance, and validate resistance. The materials will be assessed using Type II augmented designs or replicated complete block designs. For the 2020/2021 season OSU will provide the Cycle III population for a second year of evaluation and will provide the Cycle IV population of doubled haploids segregating for resistance to multiple diseases (stripe rust, TTKK stem rust, scald, Fusarium Head Blight, and/or leaf rust). Data and analyses will be posted at http://barleyworld.org/barley-stripe-rust-bsr. When sufficient data are generated, manuscripts will be submitted for publication in peer-reviewed journals.