Samples are collected from animals from all over the U.S.

Shorthorn cows in Nebraska

 

Upon arrival, sample viability is evaluated using computer assisted sperm analysis (CASA)

Example of filled, sealed and labeled straws ready to be cryopreserved

Information about sample provenance and semen storage location is entered into the Animal-GRIN database

Navajo Churro sheep in Arizona

Semen samples are cooled to 5C and cryoprotectants are added.  Samples are then packaged with an automated straw filler and labeler.

Straws are cooled to -140C using a programmed cooling apparatus, and then they are plunged into LN2

Once samples have received proper cooling treatments, they are placed in cryovats for long term storage