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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Foodborne Toxin Detection and Prevention Research » People & Locations » Bradley Hernlem (Brad)
Bradley Jay Hernlem (Brad)
Foodborne Toxin Detection and Prevention Research
Chemical Engineer

Phone: (510) 559-5937
Fax: (510) 559-6429

USDA, ARS, PWA, WRRC-FTDP
800 BUCHANAN ST
ALBANY , CA 94710
Projects
Advance the Development of Technologies for Detecting and Determining the Stability and Bioavailability of Toxins that Impact Food Safety and Food Defense
In-House Appropriated (D)
  Accession Number: 430056

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Alternative to animal use for detecting biologically active Staphylococcal enterotoxin type A -
Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2018. Alternative to animal use for detecting biologically active Staphylococcal enterotoxin type A. Toxins. 10:540. https://doi.org/10.3390/toxins10120540.
Sensitive low-cost CCD-based detector for determination of UV-LED water microbial disinfection -
Rasooly, R., Magoz, Z., Luo, J., Do, P.M., Hernlem, B.J. 2018. Sensitive low-cost CCD-based detector for determination of UV-LED water microbial disinfection. Desalination and Water Treatment. 118:120-125. https://doi.org/10.5004/dwt.2018.22667.
Low cost bioluminescence imaging as an alternative to in vivo bioassays for quantifying biologically active staphylococcal enterotoxin type E -
Rasooly, R., Do, P.M., Hernlem, B.J. 2017. Low cost bioluminescence imaging as an alternative to in vivo bioassays for quantifying biologically active staphylococcal enterotoxin type E. Sensors and Actuators B: Chemical. 259:357-393. https://doi.org/10.1016/j.snb.2017.12.079.
TCR-Vß8 as alternative to animal testing for quantifying active SEE -
Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2017. TCR-Vß8 as alternative to animal testing for quantifying active SEE. Journal of Environmental and Analytical Toxicology. 7(6):527. https://doi.org/10.4172/2161-0525.1000527.
Interleukin 2 secretion by T cells for detection of biologically active Staphylococcal enterotoxin type E Reprint Icon -
Rasooly, R., Do, P.M., Hernlem, B.J. 2017. Interleukin 2 secretion by T cells for detection of biologically active Staphylococcal enterotoxin type E. Journal of Food Protection. 80(11):1857-1862. https://doi.org/10.4315/0362-028X.JFP-17-196.
Rapid cell-based assay for detection and quantification of active staphylococcal enterotoxin type D Reprint Icon -
Rasooly, R., Do, P.M., Hernlem, B.J. 2017. Rapid cell-based assay for detection and quantification of active staphylococcal enterotoxin type D. Journal of Food Science. doi: 10.1111/1750-3841.13634.
Generation of octoploid switchgrass by seedling treatment with mitotic inhibitors -
Yoon, S., Aucar, S., Hernlem, B.J., Edme, S.J., Sarath, G., Blumwald, E., Tobias, C.M. 2016. Generation of octoploid switchgrass by seedling treatment with mitotic inhibitors. BioEnergy Research. doi: 10.1007/s12155-016-9795-2.
Generation of Neo Octaploid Switchgrass -
Yoon, S., Blumwald, E., Mitchell, R., Gautam, S., Palmer, N., Edme, S., Hernlem, B.J., Aucar, S., Tobias, C.M. 2016. Generation of Neo Octaploid Switchgrass. Meeting Abstract. 5th Pan-American Congress on Plants and BioEnergy, Santa Fe, NM, August 4-7, 2016.
Sensitive, rapid, quantitative and in vitro method for the detection of biologically active staphylococcal enterotoxin type E Reprint Icon -
Rasooly, R., Do, P.M., Hernlem, B.J. 2016. Sensitive, rapid, quantitative and in vitro method for the detection of biologically active staphylococcal enterotoxin type E. Toxins. 8(5):150. doi: 10.3390/toxins8050150.
Low cost quantitative digital imaging as an alternative to qualitative in vivo bioassays for analysis of active aflatoxin B1 Reprint Icon -
Rasooly, R., Do, P.M., Hernlem, B.J. 2016. Low cost quantitative digital imaging as an alternative to qualitative in vivo bioassays for analysis of active aflatoxin B1. Journal of Agricultural and Food Chemistry. 80:405-410.
New Stx2e monoclonal antibodies for immunological detection and distinction of Stx2 subtypes Reprint Icon -
Skinner, C.B., Patfield, S.A., Hernlem, B.J., He, X. 2015. New Stx2e monoclonal antibodies for immunological detection and distinction of Stx2 subtypes. PLoS One. 10(7):e0132419.
Plant compounds enhance assay sensitivity for detection of active bacillus cereus toxin Reprint Icon -
Rasooly, R., Hernlem, B.J., He, X., Friedman, M. 2015. Plant compounds enhance assay sensitivity for detection of active bacillus cereus toxin. Toxins. 7(3):835-845. doi: 10.3390/toxins7030835.
Intracellular trehalose and sorbitol synergistically promoting cell viability of a biocontrol yeast Pichia anomala for aflatoxin reduction Reprint Icon -
Hua, S.T., Hernlem, B.J., Yokoyama, W.H., Sarreal, S.L. 2015. Intracellular trehalose and sorbitol synergistically promoting cell viability of a biocontrol yeast Pichia anomala for aflatoxin reduction. World Journal of Microbiology and Biotechnology. doi: 10.1007/s11274-015-1824-3..
Sensitive detection of active Shiga toxin using low cost CCD based optical detector Reprint Icon -
Rasooly, R., Balsam, J., Hernlem, B.J., Rasooly, A.J. 2015. Sensitive detection of active Shiga toxin using low cost CCD based optical detector. Biosensors and Bioelectronics. 68:705-711.
Quantitative analysis of Staphylococcus enterotoxin A by differential expression of IFN-gamma in splenocyte and CD4+ T-cells Reprint Icon -
Rasooly, R., Hernlem, B.J. 2014. Quantitative analysis of Staphylococcus enterotoxin A by differential expression of IFN-gamma in splenocyte and CD4+ T-cells. Sensors. 14:8869-8876 DOI:10.3390/s140508869.
Rapid detection of predation of Escherichia coli O157:H7 and sorting of bacterivorous tetrahymena by flow cytometry -
Ravva, S.V., Hernlem, B.J., Sarreal, C.Z. 2014. Rapid Detection of predation of Escherichia coli O157:H7 and sorting of bacterivorous tetrahymena by flow cytometry. Frontiers in Cellular and Infection Microbiology. DOI:10.3389/fcimb.2014.000057.
Microwave heating inactivates Shiga Toxin (Stx2) in reconstituted fat-free Milk and adversely affects the nutritional value of cell culture medium Reprint Icon -
Rasooly, R., Hernlem, B.J., He, X., Friedman, M. 2014. Microwave heating inactivates Shiga Toxin (Stx2) in reconstituted fat-free Milk and adversely affects the nutritional value of cell culture medium. Journal of Agricultural and Food Chemistry. 62:3301-3305 DOI.org/10.1021/jf500278a.
Non-linear relationships between aflatoxin B1 levels and the biological response of monkey kidney vero cells Reprint Icon -
Rasooly, R., Hernlem, B.J., He, X. 2013. Non-linear relationships between aflatoxin B1 levels and the biological response of monkey kidney vero cells. Toxins. 5:1447-1461. doi:10.3390/toxins5081447.
Low levels of aflatoxin B1, ricin and milk enhance recombinant protein production in mammalian cells Reprint Icon -
Rasooly, R., Hernlem, B.J., Friedman, M. 2013. Low levels of aflatoxin B1, ricin and milk enhance recombinant protein production in mammalian cells. PLoS One. 8(8):e71682. doi:10.1371/journal.pone.0071682.
TNF as biomarker for rapid quantification of active Staphylococcus enterotoxin A in food -
Rasooly, R., Hernlem, B.J. 2012. TNF as biomarker for rapid quantification of active Staphylococcus enterotoxin A in food. Sensors. 12:5978-5985. doi:10.3390/s120505978.
Liquid formulations sustaining the viability of a superior yeast strain Pichia anomala WRL-076 for mycotoxin control -
Hua, S.T., Hernlem, B.J., Yokoyama, W.H., Parfitt, D.E., Sarreal, S.L. 2012. Liquid formulations sustaining the viability of a superior yeast strain Pichia anomala WRL-076 for mycotoxin control. American Society for Microbiology General Meeting. [abstract].
Techniques for rapid detection and quantification of active foodborne Staphylococcus Enterotoxin(Abstract) -
Hernlem, B.J., Rasooly, R. 2012. Techniques for rapid detection and quantification of active foodborne Staphylococcus Enterotoxin(Abstract). Meeting Abstract. Poster no. B266.
Bacterial communities in urban aerosols collected with wetted-wall cyclonic samplers and seasonal fluctuations of live and culturable airborne bacteria -
Ravva, S.V., Hernlem, B.J., Sarreal, C.Z., Mandrell, R.E. 2012. Bacterial communities in urban aerosols collected with wetted-wall cyclonic samplers and seasonal fluctuations of live and culturable airborne bacteria. Journal of Environmental Monitoring. 14(2):473-481. doi: 10.1039/clem10573d.
CD154 as a potential early molecular biomarker for rapid quantification analysis of active Staphylococcus enterotoxin A -
Rasooly, R., Hernlem, B.J. 2011. CD154 as a potential early molecular biomarker for rapid quantification analysis of active Staphylococcus enterotoxin A. FEMS Immunology and Medical Microbiology. 64(2):169-174.
Auto-presentation of Staphylococcal enterotoxin A by mouse CD4+ T cells -
Rasooly, R., Do, P.M., Hernlem, B.J. 2011. Auto-presentation of Staphylococcal enterotoxin A by mouse CD4+ T cells. Open Journal of Immunology. 1(1):8-14. doi:10.4236/oji.2011.11002.
Fluorescent viability stains to probe the metabolic status of aflatoxigenic fungus in dual culture of Aspergillus flavus and Pichia anomala -
Hua, S.T., Brandl, M., Hernlem, B.J., Eng, J.G., Sarreal, S.L. 2011. Fluorescent viability stains to probe the metabolic status of aflatoxigenic fungus in dual culture of Aspergillus flavus and Pichia anomala. Mycopathologia. 171(2):133-138.
Vitality Stains and Real Time PCR Studies to Delineate the Interactions of Pichia anomala and Aspergillus flavus -
Hua, S.T., Brandl, M., Hernlem, B.J., Sarreal, S.L. 2010. Vitality Stains and Real Time PCR Studies to Delineate the Interactions of Pichia anomala and Aspergillus flavus. American Society for Microbiology General Meeting, San Diego, CA, May 23-27, 2010.
Dihaploid stocks for switchgrass isolated by a screening approach -
Young, H.A., Hernlem, B.J., Anderton, A.L., Lanzatella-Craig, C., Tobias, C.M. 2010. Dihaploid stocks for switchgrass isolated by a screening approach. BioEnergy Research. 3:305-313.
Dual fluorochrome flow cytometric assessment of yeast viability -
Hernlem, B.J., Hua, S.T. 2010. Dual fluorochrome flow cytometric assessment of yeast viability. Current Microbiology. 61:57-63.
Development of monoclonal antibodies specific for Ricinus agglutinins -
Brandon, D.L., Hernlem, B.J. 2009. Development of monoclonal antibodies specific for Ricinus agglutinins. Food and Agricultural Immunology. 20(1):11-22
Application of flow cytometry and cell sorting to the bacterial analysis of environmental aerosol samples -
Hernlem, B.J., Ravva, S.V. 2007. Application of flow cytometry and cell sorting to the bacterial analysis of environmental aerosol samples. Journal of Environmental Monitoring. 2007(9) 1317-1322.
ELECTROLYTIC DISINFECTION OF ESCHERICHIA COLI AND COLIFORM BACTERIA IN A BATCH CELL WITH DSA ELECTRODES -
Hernlem, B.J. 2006. Electrolytic disinfection of escherichia coli and coliform bacteria in a batch cell with dsa electrodes [Abstract]. ISAC XXIII International Congress. Abstract #229; Poster # P83.
COMBINATION OF CHLORINE-FREE ELECTROLYTIC AND PHOTOCHEMICAL METHODS FOR STERILIZATION OF CONTAMINATED WATERS -
Barashkov, N.N., El Fenni, M., Eisenberg, D., Eisenberg, S., Lam, L., Hernlem, B.J. Combination of chlorine-free electrolytic and photochemical methods for sterilization of contaminated waters [Abstract]. The 232nd ACS National Meeting. Paper ENVR 161.
ELECTROLYTIC DESTRUCTION OF UREA IN DILUTE CHLORIDE SOLUTION USING DSA ELECTRODES IN A RECYCLED BATCH CELL -
Hernlem, B.J. 2005. "Electrolytic Destruction of Urea in Dilute Chloride Solution using DSA Electrodes in a Recycled Batch Cell." Water Research. 39(11):2245-2252
ELECTROFLOTATION OF DAIRY MANURE LAGOON WATER: CHEMICAL & MICROBIOLOGICAL EFFECTS -
Hernlem, B.J. 2004. Electroflotation of dairy manure lagoon water: chemical & microbiological effects. Meeting Abstract.
DISINFECTION AND SOLIDS REMOVAL OF POULTRY CHILLER WATER BY ELECTROFLOTATION -
Tsai, L.-S., Hernlem, B.J., Huxsoll, C.C. 2002. Disinfection and Solids Removal of Poultry Chiller Water by Electroflotation. Journal of Food Science. 67(6):2160-2164.
CHLORINE GENERATION AND DISINFECTION BY ELECTROFLOTATION -
Hernlem, B.J., Tsai, L.-S. 2000. Chlorine Generation and Disinfection by Electroflotation. Journal of Food Science. 65(4):834-837.
COMBINED ELECTROFLOTATION AND DISINFECTION IN FOOD PROCESSING -
Hernlem, B.J., Tsai, L.-S., Huxsoll, C., Robertson, G. 2000. Combined Electroflotation and Disinfection in Food Processing. Paper Number 74-12, presented at the 2000 Institute of Food Technologists Annual Meeting, June 10-14, 2000, Dallas, TX. Available: http://ift.confex.com/ift/2000/techprogram/paper_4502.htm.
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