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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Publications at this Location » Publication #98926
Title: ISOZYME STUDIES IN SOYBEAN BY STARCH GEL ELECTROPHORESIS

Author
item RAM, CHANDGI - SEED TECHNL. CENTRE,INDIA
item AMBERGER, L - IOWA STATE UNIVERSITY
item Palmer, Reid

Submitted to: Proceedings National Seed Seminar
Publication Type: Abstract Only
Publication Acceptance Date: 4/7/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Cultivar identification and techniques to assess cultivar purity are essential to commercial seed production and certification. Isozyme electro-phoresis, a procedure which differentially migrates polymorphic enzyme proteins through a gelatinous matrix in an electric field has emerged as a useful technique for cultivar identification. Starch gel electrophoresis has the added advantage of analyzing several isozymes in a single run. Nine isozymes added advantage of analyzing several isozymes in a single run. Nine isozymes viz., Aconitase, Acid Phosphatase, Diaphorase, Endopeptidase, Malate Dehydrogenase, Isocitrate Dehydrogenase, Malic Enzyme, Phosphoglucomutase and Phosophoglucose Isomerase were analyzed by starch gel electrophoresis. Isozyme analysis revealed that the soybean varieties had considerable variation w.r.t. isozymes which could be used for varietal identification of soybean. These results suggest that sufficient variability is present in soybean varieties to allow the use of isozyme analysis as a system for cultivar identification thus complimenting the traditional method currently used.