Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/1999
Publication Date: N/A
Interpretive Summary: An interpretive summary is not required for this abstract.
Technical Abstract: Porcine stromal vascular cells (S/V cells) differentiate into adipocytes upon adequate hormonal stimulation. Porcine S/V cells were differentiated in vitro with or without fatty acids to determine the effect of fatty acids on transcripts for peroxisome proliferator-activated receptor gamma (PPAR gamma), CAAT/enhancer binding protein alpha (C/EBP alpha), adipocyte fatty acid binding protein (aP2), and lipoprotein lipase (LPL) by northern analysis using porcine riboprobes. At day 0 of differentiation, transcripts for PPAR gamma and CEBP alpha were at >35% of the day 10 levels. The mRNA levels for aP2 and LPL increased gradually during differentiation. Addition of 100 muM oleic acid (18:1) for 5 days significantly increased adipocyte differentiation and the mRNA levels of PPAR gamma, C/EBP alpha, aP2, and LPL (about twofold). Other medium- and long-chain fatty acids were less active, except linoleic acid (18:2), which increased the aP2 level. In contrast, 100 muM conjugated linoleic acid reduced the mRNA levels for PPA gamma, C/EBP alpha, and LPL (about 50%). These results indicate selected fatty acids modulate porcine adipocyte differentiation and the transcripts for differentiation related proteins such as PPAR gamma, CEBP alpha, aP2 and LPL.