Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/30/1998
Publication Date: N/A
Interpretive Summary: Macrophages are an immune cell population that perform many important functions that include ingesting and killing of microbes and tumor cells, production of cell communication molecules (cytokines), and the processing and presentation of foreign substances to other immune cells. Macrophages begin in the bone marrow and differentiate into a blood cell population called monocytes. When the monocytes reach specific tissues they further differentiate into macrophages specific to that tissue. The differentiation is marked by cell surface markers which correlate with functional changes. Two antibodies were identified that recognize a cell surface marker that is unique to mature macrophages. These antibodies will enable researchers to better study the activational state of macrophages in pigs.
Technical Abstract: Two monoclonal antibodies (mAb) within cluster M4 of the myeloid section of the Second International Swine CD Workshop, C4 (No. 144) and PM18-7 (No. 192), showed reactivity with thymocytes and among cells of myelomonocytic origin with mature macrophages but not with monocytes and granulocytes. Both mAb recognize a protein showing two bands of 205 kDa and 130 kDa under rboth reducing and non-reducing conditions. Although epitope mapping with these mAb could not be performed, this cluster received the SWC9 designation