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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Infectious Bacterial Diseases Research » Research » Publications at this Location » Publication #92081
Title: RETROSPECTIVE COMPARISON OF HISTOPATHOLOGY AND IMMUNOHISTOCHEMISTRY FOR DIAGNOSING OVINE SCRAPIE

Author
item JENNY, ALLEN - APHIS,NVSL,AMES,IOWA
item TAYLOR, WILLIAM - APHIS,NVSL,AMES,IOWA
item Miller, Janice
item DAVIS, ARTHUR - APHIS,NVSL,AMES,IOWA

Submitted to: American Association of Veterinary Laboratory Diagnosticians
Publication Type: Abstract Only
Publication Acceptance Date: 7/10/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Histopathologic diagnoses were compared with the results obtained by hydrated autoclaving immunohistochemistry on brain for the abnormal form of the prion protein (PrP**res) on samples from more than 350 sheep showing clinical signs suggestive of CNS disease or progressive weight loss. When histopathology findings were consistent with scrapie, immunohisto- chemistry results were positive in over 99 percent of the cases. When histopathology findings were suggestive of scrapie, immunohistochemistry results were positive for PrP**res in over 80 percent of the animals. In cases where no diagnosis was established but some lesions characteristic of scrapie were found, i.e., vacuolated neurons and/or spongiform change in the grey matter neuropil, immunohistochemistry results were positive for PrP**res in over 50 percent of animals. The significance of the findings in a scrapie control or eradication program will be discussed.