Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: 1/12/1998
Publication Date: N/A
Technical Abstract: Source of clones. Porcine genomic DNA library was screened for microsatellites as described by Stone et al. (1997). Primers were developed as described by Rohrer et al. (1994). Description of polymorphisms. Three alleles ranging from 123 to 131 bases were detected in the MARC swine reference population for KS3 and seven alleles ranging from 273 to 305 bases were detected for KS4. Mendelian inheritance of each allele was confirmed in seven fullsib families of the MARC swine reference population. Four parents of the reference population were heterozygous for KS3 while eight out of nine parents were heterozygous for KS4. Chromosome location. There were 43 informative meioses for KS3 and 152 informative meioses for KS4. Two-point linkage analyses were conducted with CRI-MAP 2.4 between these two markers and all markers present in the MARC genome database. The most significant linkages detected were with S0031 (LOD=8.43 & theta=.00) & S0087 (LOD=30.38 & theta=.03) for KS3 and KS4, respectively. Multipoint linkage analyses indicated that the most likely position for KS3 was at position 110.4 cM and 59.8 cM for KS4 on the map published by Rohrer et al. (1996). Figure 1 presents an ideogram of the updated linkage group. Comments. These two markers may be useful to scientists mapping QTL on porcine chromosome 6. Both markers were easy to score. KS3 was not very informative and will likely have limited utility for QTL mapping. However, KS3 was informative in all F1 Meishan-White Composite sows indicating it may be quite useful in populations containing Meishan by western breed cross. KS4 was quite informative in the parents of the reference population.