Submitted to: American Society of Sugarbeet Technologists
Publication Type: Abstract Only
Publication Acceptance Date: 2/1/1997
Publication Date: N/A
Technical Abstract: Sugarbeet tissues contain a wide variety of proteins, ranging from structural to enzymatic, which could be used as genetic or developmental markers. Extracts of tissues can be directly applied to a protein denaturing solid matrix sieve and separated according to molecular weight in a voltage gradient, a rapid and reliable technique known as SDS-polyacrylamide electrophoresis (SDS-PAGE). The resulting separation of proteins can be used to gauge the complexity of proteins expressed in different tissues, such as leaf, stem or root, as well as to follow changes in protein composition during development, for instance between vegetative and reproductive phases. In certain cases, protein profiles can serve as a 'fingerprint'. We have begun to examine sugarbeet tissues for differences in total protein profiles between East Lansing breeding lines that may be useful genetic or developmental markers. Specifically, we are examining three pairs of cms-lines and their matching O-types as well as one near O-type line. We are particularly interested in (1) determining the protein profiles in roots and whether these may correlate with amino-N in sugarbeet juice and (2) whether SDS-PAGE can supplement the indexing of cms maintainer lines.