Loop-mediated isothermal amplification (LAMP) for the detection and quantification of fungal phytopathogens

Authors: SIC, WILY - Purdue University; GOMEZ, SANDRA - Purdue University; Goodwin, Stephen - Steve

Submitted to: CRC Press
Publication Type: Book / Chapter
Publication Acceptance Date: 5/2/2024
Publication Date: N/A
Citation: N/A

Interpretive Summary: N/A

Technical Abstract: Accurate identification of fungal pathogens that are causal agents of plant diseases is necessary to make the right decisions about disease management. Loop-mediated isothermal amplification (LAMP) is emerging as a simple and highly sensitive diagnostic tool for the early detection of fungal plant pathogens. LAMP is an isothermal technique for the amplification of nucleic acids. The technique uses four to six primers to amplify a target-specific DNA sequence and generates DNA products with stem-loop and cauliflower-like structures with multiple loops. The amplified products are easily detected by a change in the turbidity of the solution, color change after adding an intercalating dye to the reaction solution, or fluorescence measurement. LAMP has been used to detect fungal phytopathogens in soybean, rice, grapes, cotton, banana, corn, and wheat, showing high specificity and sensitivity. Also, a variety of portable devices for real-time LAMP applications has been developed showing potential for the detection of plant pathogens in field conditions. One caveat is that this technique requires an extensive optimization process to be effective under field conditions due to cross contamination.