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ARS Home » Pacific West Area » Riverside, California » National Clonal Germplasm Repository for Citrus » Research » Publications at this Location » Publication #409355

Research Project: Conservation, Management and Distribution of Citrus and Date Genetic Resources and Associated Information

Location: National Clonal Germplasm Repository for Citrus

Title: Navigating the genetic bottleneck of HLB: exploring the genomes of Australian limes for development of disease resistance

item SINGH, K - University Of California, Riverside
item HUFF, M - University Of Tennessee
item LIU, JIANYANG - Department Of Energy
item PARK, J - Texas A&M University
item RICKMAN, T - University Of Tennessee
item Keremane, Manjunath
item Krueger, Robert
item ZHENG, P - Washington State University
item HUMMAN, J - Washington State University
item KUNTA, M - Texas A&M University
item ROOSE, M - University Of California, Riverside
item MAIN, D - Washington State University
item STANTON, M - University Of Tennessee
item Dardick, Christopher - Chris
item RAMADUGU, C - University Of California, Riverside

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/8/2023
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The citrus industry needs a solution against the scourge of huanglongbing (HLB). Australian limes such as Citrus australasica, C. inodora and C. glauca possess valuable disease-resistant traits against HLB. To identify the resistant genes and understand the basis of resistance in the Australian limes, we developed high-quality, de novo, haplotype genome assemblies. The genome sizes range from 300 Mb (C. inodora) to 337 Mb (C. australasica) and 377 Mb (C. glauca). All three genome sequences were scaffolded into nine large, chromosome-scale scaffolds that constitute 86% – 91% of total genome. Synteny relationships of the three genomes showed variation in chromosomes 2, 3, 4, 6 and 9 when compared to C. clementina. Genome annotations identified 25,461 genes in C. australasica, 27,665 in C. inodora, and 30,067 in C. glauca. There were 668 R-genes in C. australasica, 404 in C. inodora, and 564 in C. glauca. We identified 47-56 Phloem protein2 genes and ~20 Callose synthase genes. In conclusion, the exploration of Australian limes has unveiled a treasure trove of genetic diversity and provides invaluable insights that hold the potential to understand HLB disease resistance.