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ARS Home » Plains Area » Lincoln, Nebraska » Agroecosystem Management Research » Research » Publications at this Location » Publication #406574

Research Project: Managing Manure as a Soil Resource for Improved Biosecurity, Nutrient Availability, and Soil Sustainability

Location: Agroecosystem Management Research

Title: Evaluation of idexx quantitray for quantification and isolation of antibiotic resistant bacteria

item Durso, Lisa
item Wells, James - Jim
item Ibekwe, Abasiofiok - Mark
item Frye, Jonathan
item Sharma, Manan
item Berry, Elaine

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 8/8/2023
Publication Date: 11/1/2023
Citation: Durso, L.M., Wells, J., Ibekwe, A.M., Frye, J.G., Sharma, M., Berry, E.D. 2023. Evaluation of idexx quantitray for quantification and isolation of antibiotic resistant bacteria. ASA-CSSA-SSSA Annual Meeting Abstracts. Available:

Interpretive Summary:

Technical Abstract: The IDEXX Colilert Quantitray system is widely used by water quality professionals, municipalities, and research scientists for water quality monitoring of Escherichia coli and total coliforms. Given this infrastructure already in place, a potential exists for widespread surveillance of antibiotic resistance of E. coli in surface waters using a modification of this method. The idea was first proposed in 1986,has been reported occasionally in the peer-reviewed literature, and anecdotal evidence suggests increasing use of this method research laboratories. Here we evaluated the method for its ability to enumerate extended spectrum beta-lactamase E. coli and tetracycline resistant E. coli, including limits of detection using an agriculturally-derived control strain, sensitivity, and specificity. The ability of the assay to detect the target organisms under a range of turbidities was also examined.