Location: Agroecosystem Management Research
Title: Evaluation of idexx quantitray for quantification and isolation of antibiotic resistant bacteriaAuthor
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Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only Publication Acceptance Date: 8/8/2023 Publication Date: 11/1/2023 Citation: Durso, L.M., Wells, J., Ibekwe, A.M., Frye, J.G., Sharma, M., Berry, E.D. 2023. Evaluation of idexx quantitray for quantification and isolation of antibiotic resistant bacteria. ASA-CSSA-SSSA Annual Meeting Abstracts. Available: https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/149118. Interpretive Summary: Technical Abstract: The IDEXX Colilert Quantitray system is widely used by water quality professionals, municipalities, and research scientists for water quality monitoring of Escherichia coli and total coliforms. Given this infrastructure already in place, a potential exists for widespread surveillance of antibiotic resistance of E. coli in surface waters using a modification of this method. The idea was first proposed in 1986,has been reported occasionally in the peer-reviewed literature, and anecdotal evidence suggests increasing use of this method research laboratories. Here we evaluated the method for its ability to enumerate extended spectrum beta-lactamase E. coli and tetracycline resistant E. coli, including limits of detection using an agriculturally-derived control strain, sensitivity, and specificity. The ability of the assay to detect the target organisms under a range of turbidities was also examined. |
