|DAHAN, JENNIFER - University Of Idaho|
|ORELLANA, GARDENIA - University Of Idaho|
|KARASEV, ALEXANDER - University Of Idaho|
Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/15/2023
Publication Date: N/A
Technical Abstract: Grapevine rupestris stem pitting-associated virus (GRSPaV) exists as a complex of multiple genetic variants associated with cultivars of Vitis vinifera L. In the United States, GRSPaV was found in grapevines in California, Washington, New York, and Tennessee; GRSPaV was never reported in Idaho. Grapevine-associated tymo-like virus (GaTLV) was reported to infect several grapevine cultivars in France. GaTLV presence in Idaho vineyards was never addressed. In September 2020, leaf and petiole samples were collected from six vineyards in two counties of Idaho. Ribodepleted total RNAs prepared from 16 samples were subjected to a HTS analysis on a NovaSeq 6000 platform, producing between 15,095,042 and 31,500,611 250-bp paired-end reads per sample. One of the samples, from an own-rooted, 38-yr old Riesling vine yielded a large, 8,700-nt contig, with 98.5% identity to the GRSPaV isolate from Canada; the same sample also yielded an 8,481-nt contig exhibiting 98.3%identity to the GRSPaV isolate from France. Three of the samples, two collected from own-rooted Chardonnay vines, and one from an own-rooted Cabernet franc vine, yielded large, 6,005 to 6,024-nt contigs exhibiting 99.0% identity to the sequence of the GaTLV from France. Presence of GRSPaV and GaTLV was confirmed in the original samples using RT-PCR with virus-specific primers and Sanger re-sequencing. GaTLV was found common in Idaho wine grapes, present in 12 out of 34 samples collected from four cultivars in five vineyards in 2020-2021. GRSPaV, on the other hand, was found only in a single cultivar in a single vineyard.