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ARS Home » Plains Area » Kerrville, Texas » Knipling-Bushland U.S. Livestock Insects Research Laboratory » Veterinary Pest Genetics Research Unit » Research » Publications at this Location » Publication #400145

Research Project: Genetics of Veterinary Pests

Location: Veterinary Pest Genetics Research Unit

Title: Horn fly transcriptomes from 10 populations from the southern United States

item Bendele, Kylie
item GUERRERO, FELIX - Retired ARS Employee
item Lohmeyer, Kimberly - Kim
item FOIL, LANE - Louisiana State University
item METZ, RICHARD - Texas Agrilife Research
item JOHNSON, CHARLES - Texas Agrilife Research

Submitted to: Data in Brief
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/22/2023
Publication Date: 5/30/2023
Citation: Bendele, K.G., Guerrero, F., Lohmeyer, K.H., Foil, L., Metz, R., Johnson, C. 2023. Horn fly transcriptomes from 10 populations from the southern United States. Data in Brief.

Interpretive Summary: Ten populations of horn flies with varying levels of pesticides were sequenced and assembled into a transcriptome for each population. These transcriptomes are from horn flies with different mechanisms of phenotypes that have been characterized for insecticide resistance. These transcriptomes are valuable tools for researchers studying these different insecticide resistance and can be used in comparative studies of the molecular mechanisms causing insecticide resistance in horn flies.

Technical Abstract: Haematobia irritans irritans (Linnaeus, 1758: Diptera: Muscidae), the horn fly, is an external parasite of penned and pastured livestock that causes a major economic impact on cattle production worldwide. Pesticides such as synthetic pyrethroids and organophosphates are routinely used to control horn flies; however, resistance to these chemicals has become a concern in several countries. To further elucidate the molecular mechanisms of resistance in horn fly populations, we sequenced the transcriptomes of ten populations of horn flies from the southern US possessing varying degrees of pesticide resistance using an Illumina paired-end HiSeq approach, followed by de novo assembly of the transcriptomes using CLC Genomics Workbench 8.0.1 De Novo Assembler using multiple kmers, and annotation using Blast2GO PRO version 5.2.5. The raw sequence reads are archived in the Sequence Read Archive (SRA) and assembled population transcriptomes in the Transcriptome Shotgun Assembly (TSA) at the National Center for Biotechnology Information (NCBI).