Location: Genetics and Animal BreedingTitle: Recent developments and future directions in meta-analysis of differential gene expression in livestock RNA-Seq
Submitted to: Frontiers in Genetics
Publication Type: Review Article
Publication Acceptance Date: 8/24/2022
Publication Date: 9/19/2022
Citation: Keel, B.N., Lindholm-Perry, A.K. 2022. Recent developments and future directions in meta-analysis of differential gene expression in livestock RNA-Seq. Frontiers in Genetics. 13. Article 983043. https://doi.org/10.3389/fgene.2022.983043.
Technical Abstract: Decreases in the costs of high-throughput sequencing technologies have led to continually increasing numbers of livestock RNA-Seq studies in the last decade. Although the number of studies has increased dramatically, most livestock RNA-Seq experiments are limited by cost to a small number of biological replicates. Meta-analysis procedures can be used to integrate and jointly analyze data from multiple independent studies. Meta-analyses increase the sample size, which in turn increase both statistical power and robustness of the results. In this work, we discuss cutting edge approaches to combining results from multiple independent RNA-Seq studies to improve livestovk transcriptomics research. We review currently published RNA-Seq meta-analyses in livestock, describe many of the key issues specific to RNA-Seq meta-analysis in livestock species, and discuss future perspectives.