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ARS Home » Southeast Area » Canal Point, Florida » Sugarcane Field Station » Research » Publications at this Location » Publication #393502

Research Project: Identification of Resistant Germplasm and Markers Associated with Resistance to Major Diseases of Sugarcane

Location: Sugarcane Field Station

Title: Identifying sugarcane orange rust resistance genes by single-cell and spatial transcriptomics

item LOU, ZILIANG - University Of Florida
item ZHOU, YUPENG - University Of Florida
item Islam, Md
item Sood, Sushma
item WANG, JIANPING - University Of Florida

Submitted to: American Society of Sugar Cane Technologists
Publication Type: Abstract Only
Publication Acceptance Date: 3/31/2022
Publication Date: 7/16/2022
Citation: Lou, Z., Zhou, Y., Islam, M.S., Sood, S.G., Wang, J. 2022. Identifying sugarcane orange rust resistance genes by single-cell and spatial transcriptomics. American Society of Sugar Cane Technologists. 33-34.

Interpretive Summary: N/A

Technical Abstract: Sugarcane (Saccharum spp.) is an important economic crop widely used as a source of sugar and biofuel. Orange rust disease, caused by Puccinia kuehnii, is one of the major diseases threatening sugarcane production in the United States. Breeding for disease-resistant cultivars is a challenge because research on sugarcane orange rust resistance is lagging, and disease resistance genes have yet to be discovered. Two sister clone lines with opposite orange rust resistance phenotype (resistant line: 540 and susceptible line: 664) were identified from an F1 hybrid population derived from a cross between CP95-1039 and CP88-1762 clones. We used single-cell and spatial transcriptomics to investigate the molecular dynamics of orange rust disease resistance of the two lines. Sugarcane plants were inoculated with orange rust spores. Leaf tissues were collected for analysis at 10 days after inoculations. The gene expression at single cell resolution was analyzed to identify candidate genes for orange rust resistance. Identified genes associated with rust resistance will be used to facilitate marker-assisted selection and breeding.