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ARS Home » Northeast Area » Washington, D.C. » National Arboretum » Floral and Nursery Plants Research » Research » Publications at this Location » Publication #390181

Research Project: Evaluation and Genetic Improvement of Woody Ornamental Landscape Plants

Location: Floral and Nursery Plants Research

Title: Genotype-independent plant transformation

item Duan, Hui
item MAREN, NATHAN - North Carolina State University
item DA, KEDONG - North Carolina State University
item YENCHO, GRIAG - North Carolina State University
item RANNEY, THOMAS - North Carolina State University
item LIU, WUSHENG - North Carolina State University

Submitted to: Horticulture Research
Publication Type: Review Article
Publication Acceptance Date: 1/23/2022
Publication Date: 2/24/2022
Citation: Duan, H., Maren, N.A., Da, K., Yencho, G.G., Ranney, T.G., Liu, W. 2022. Genotype-independent plant transformation. Horticulture Research. pages 9.

Interpretive Summary:

Technical Abstract: Plant transformation and regeneration remain highly species- and genotype-dependent. Conventional hormone-based plant regeneration via somatic embryogenesis or organogenesis are tedious, time-consuming, and high skill- and experience-demanding. Over the last 40 years, significant strides have been made to elucidate the molecular mechanisms underlying the induction and formation of somatic embryos, meristems, or other organs. These pioneering studies led to a better understanding of the key steps and factors involved in plant regeneration. These studies also resulted in the identification of crucial growth and developmental regulator genes that dramatically improve regeneration efficiency, shorten transformation time, and make transformation of recalcitrant genotypes possible. These regulator genes offer great potential to develop innovative genotype-independent genetic transformation methods for various plant species, including specialty crops. They also provide innovative strategies for plant transformation that may avoid the use of hormones, antibiotics, selectable marker genes, or tissue culture. As an enabling technology, the use of these regulator genes in plant biotechnology is expected to promote the application of advanced breeding technologies such as genetic engineering and gene editing for trait improvement in transformation-recalcitrant crops and cultivars. This review will discuss the recent advances in the use of these genes in plant transformation and regeneration, and their potential to facilitate genotype-independent plant transformation and regeneration.