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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Foodborne Toxin Detection and Prevention Research » Research » Publications at this Location » Publication #389036

Research Project: Novel Methods for the Mitigation of Human Pathogens and Mycotoxin Contamination of High Value California Specialty Crops

Location: Foodborne Toxin Detection and Prevention Research

Title: Antifungal activity of Antrodia cinnamomea mushroom extracts: Efficacy and resistance

item Kim, Jong Heon
item Tam, Christina
item Chan, Kathleen - Kathy
item MAHONEY, NOREEN - Former ARS Employee
item CHENG, LUISA - Former ARS Employee
item Friedman, Mendel
item LAND, KIRKWOOD - University Of The Pacific

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/2/2021
Publication Date: 11/2/2021
Citation: Kim, J., Tam, C.C., Chan, K.L., Mahoney, N., Cheng, L.W., Friedman, M., Land, K.M. 2021. Antifungal activity of Antrodia cinnamomea mushroom extracts: Efficacy and resistance. Meeting Abstract.

Interpretive Summary:

Technical Abstract: Edible mushrooms are a sustainable source of unique bioactive metabolites, including antifungal compounds. We investigated antifungal activities of the water or methanol extracts of the medicinal mushrooms Antrodia cinnamomea, Agaricus blazei and Ganoderma spp. against yeast (Candida albicans) and filamentous (Aspergillus fumigatus) fungal pathogens. In the zone of inhibition bioassay, only the methanol extracts of A. cinnamomea (AcM) exhibited potent antifungal activity against C. albicans and A. fumigatus. Resistance testing also indicated that two Penicillium expansum antioxidant mutants were tolerant to the conventional antifungal agent, fludioxonil. P. expansum antioxidant mutants also showed tolerance to AcM. Results indicated that the AcM antifungal action is mediated via the normal antioxidant signaling system present in fungi, where the antioxidant mutants escape the toxicity triggered by AcM. In a benzoic analog bioassay, P. expansum mutants showed similar type of tolerance to the benzoic derivative, thus indicating the presence of natural ingredients in AcM, such as benzoic compounds, could negatively affect the efficacy of AcM when antioxidant mutants are targeted. Thus, AcM could be developed as an effective antifungal agent; however, caution should be exercised in its use of AcM as an antifungal compound so as not to trigger the development of resistance of antioxidant mutants to the treatment.