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ARS Home » Midwest Area » Madison, Wisconsin » U.S. Dairy Forage Research Center » Cell Wall Biology and Utilization Research » Research » Publications at this Location » Publication #387342

Research Project: Improving Utilization of Forages in Integrated Dairy Production Systems to Enhance Sustainable Farming Systems and Food Security

Location: Cell Wall Biology and Utilization Research

Title: Streptomyces apricus sp. nov. isolated from soil

item HARIHARAN, JANANI - Cornell University
item CHOUDOIR, MALLORY - University Of Massachusetts
item DIEBOLD, PETER - Cornell University
item Panke-Buisse, Kevin
item BUCKLEY, DANIEL - Cornell University

Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/24/2021
Publication Date: 1/17/2022
Citation: Hariharan, J., Choudoir, M.J., Diebold, P., Panke-Buisse, K., Buckley, D.H. 2022. Streptomyces apricus sp. nov. isolated from soil. International Journal of Systematic and Evolutionary Microbiology. 72(1).

Interpretive Summary: Species of the genus Streptomyces of the phylum Actinobacteria are found in a wide range of habitats and are common in soils worldwide. Filamentous actinomycetes are responsible for producing over two-thirds of known antibiotics, with Streptomyces producing a majority of these compounds. Streptomyces have been noted for their high rates of homologous recombination, which can complicate species identification. In addition, the genetic diversity and secondary metabolite genes of Streptomyces species can diverge along geographical gradients. These results suggest that while Streptomyces are capable of rapid dispersal, evolutionary and ecological processes can produce regionally defined species pools. Localized speciation suggests the presence of additional uncharacterized potentially useful species and strains.

Technical Abstract: A novel Streptomyces strain, SUN51, was isolated from soils in Wisconsin, USA, as part of a Streptomyces biogeography survey. Genome sequencing revealed that this strain had less than 90 percent average nucleotide identity (ANI) to type species of Streptomyces. SUN51was most closely related to Streptomyces dioscori A217 (99.5 percent 16S rRNA identity, 89.4 percent ANI). Genome size was estimated at 8.81 Mb, and genome DNA GC content was 72 percent. The strain possessed the cellular fatty acids anteiso-C15:0, iso-C16:0, and 16:1 w7c, anteiso-C17:0, iso-C14:0 and C16:0. SUN51 contained polar lipids of phosphatidic acid, phosphatidyl ethanolamine, phosphatidyl glycerol, and diphosphatidyl glycerol. The strain can grow on a broad range of carbon sources and tolerate temperatures of up to 40 degrees C. The results of the polyphasic approach confirmed that this isolate represents a novel species of the genus Streptomyces, for which the name Streptomyces apricus is proposed.