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ARS Home » Northeast Area » Washington, D.C. » National Arboretum » Floral and Nursery Plants Research » Research » Publications at this Location » Publication #387182

Research Project: Detection, Identification, and Characterization of New and Emerging Viral and Bacterial Diseases of Ornamental Plants

Location: Floral and Nursery Plants Research

Title: Construction of full-length infectious cDNA clones of two Korean isolates of Turnip mosaic virus breaking resistance in Brassica napus

item SONG, ZHENG-XING - Chungnam National University
item SEO, EUN-YOUNG - Chungnam National University
item HU, WEN-XING - Chungnam National University
item JEONG, JONG-HYEON - Chungnam National University
item MOON, JAE SUN - Korea Research Institute Of Bioscience And Biotechnology
item KIM, KANG-HEE - Chungnam National University
item EOM, WON-SEOB - Chungnam National University
item CHO, IN-SOOK - Rural Development Administration - Korea
item Hammond, John
item LIM, HYOUN-SUB - Chungnam National University

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/23/2021
Publication Date: 3/8/2022
Citation: Song, Z., Seo, E., Hu, W., Jeong, J., Moon, J., Kim, K., Eom, W., Cho, I., Hammond, J., Lim, H. 2022. Construction of full-length infectious cDNA clones of two Korean isolates of Turnip mosaic virus breaking resistance in Brassica napus. Archives of Virology.

Interpretive Summary: Plant virus infections cause reductions of yield and quality in many crops, and may be spread within and between crops by vectors, including insects, or by mechanical transmission in the course of horticultural operations. New virus isolates may arise by mutation, recombination, or by movement into the crop from external sources, often resulting from long-distance movement of windblown insect vectors such as aphids, or via transmission through infected seeds. Scientists in Korea discovered new isolates of turnip mosaic virus affecting canola (Brassica napus), the first time this virus has been reported infecting canola in Korea. The new isolates were shown to belong to the World-B group of turnip mosaic virus isolates, similar to isolates previously characterized in China, whereas the isolates previously characterized in Korea belong to the Basal-BR group, and differ in pathogenicity from the new isolates. Detection of turnip mosaic virus infection of canola in Korea suggests the potential for losses to the canola crop, and the need to identify canola germplasm or varieties with resistance to turnip mosaic virus to limit such potential losses.

Technical Abstract: In this work, two new Turnip mosaic virus (TuMV) strains (Canola-12 and Canola-14) overcoming resistance in canola (Brassica napus) were isolated from a B. napus sample which showed typical TuMV-like symptoms and was collected from Gimcheon city, South Korea in 2020. Complete genomes and infectious clones of each isolate were obtained. Phylogenetic analysis indicated that the strains isolated from canola belonged to the World-B group. Both infectious clones which were driven by 35S and T7 promoters induced systemic symptoms on Nicotiana benthamiana and B. napus. To our knowledge, this is the first report of TuMV infecting B. napus in South Korea.