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ARS Home » Midwest Area » Madison, Wisconsin » U.S. Dairy Forage Research Center » Cell Wall Biology and Utilization Research » Research » Publications at this Location » Publication #385979

Research Project: Investigating Microbial, Digestive, and Animal Factors to Increase Dairy Cow Performance and Nutrient Use Efficiency

Location: Cell Wall Biology and Utilization Research

Title: Truncation of IFT80 causes early embryonic loss in cattle

item ORTEGA, M - University Of Missouri
item Bickhart, Derek
item CLARK, KELSEY - University Of Missouri
item Null, Daniel
item Hutchison, Jana
item McClure, Jennifer
item COLE, JOHN - Former ARS Employee

Submitted to: Journal of Dairy Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/31/2022
Publication Date: N/A
Citation: N/A

Interpretive Summary: The US dairy industry is reliant on cow fertility for high levels of milk production. Modern study of genetics has uncovered many naturally-occurring genetic variants that can impact this fertility. One of these variants which impacted 1.66% of the US Holstein cow population was identified. Using CRISPR-Cas technology, we confirmed the impact of this variant and can now track it in the US dairy herd.

Technical Abstract: Recessive alleles represent a substantial risk in populations that have undergone significant bottleneck events. Analyzing millions of genotypes and hundreds of sequenced bulls, we identified one pernicious recessive variant that achieved 1.66% frequency in the US Holstein population and confirmed loss of embryonic development via CRISPR-Cas9 editing. In the process, we identified the developmental significance of two protein domains of the IFT80 gene in the embryonic development of cattle.