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Research Project: Intervention Strategies to Control Endemic and New and Emerging Viral Diseases of Swine

Location: Virus and Prion Research

Title: Harness organoid models for virological studies in animals: A cross-species perspective

Author
item SANG, YONGMING - TENNESSEE STATE UNIVERSITY
item Miller, Laura
item NELLI, RAHUL - IOWA STATE UNIVERSITY
item GIMÉNEZ-LIROLA, LUIS - IOWA STATE UNIVERSITY

Submitted to: Frontiers in Microbiology
Publication Type: Review Article
Publication Acceptance Date: 8/27/2021
Publication Date: 9/16/2021
Citation: Sang, Y., Miller, L.C., Nelli, R.K., Giménez-Lirola, L.G. 2021. Harness organoid models for virological studies in animals: A cross-species perspective. Frontiers in Microbiology. 12. Article 725074. https://doi.org/10.3389/fmicb.2021.725074.
DOI: https://doi.org/10.3389/fmicb.2021.725074

Interpretive Summary: Organoids are derived from three-dimensional culture of stem cells under certain conditions. Organoids have broadened virological studies in the human context, particularly in recent use for COVID19 research. This review examines the status and potential for cross-species applied organotypic culture in validating emerging animal, particularly zoonotic, viruses in domestic and wild animals.

Technical Abstract: Animal models and cell culture in vitro are primarily used in virus and antiviral immune research. Whereas the limitation of these models to recapitulate the viral pathogenesis in humans has been made well aware, it is also imperative to introduce more efficient systems to validate emerging viruses in both domestic and wild animals. Organoids ascribe to representative miniatures of organs (i.e., mini-organs), which are derived from three-dimensional culture of stem cells under respective differential conditions mimicking endogenous organogenetic niches. Organoids have broadened virological studies in the human context, particularly in recent use for COVID19 research. This review examines the status and potential for cross-species applied organotypic culture in validating emerging animal, particularly zoonotic, viruses in domestic and wild animals.