Location: National Clonal Germplasm Repository for CitrusTitle: Study and detection of citrus viroids in woody hosts
|VIDALAKIS, GEORGIOS - University Of California|
Submitted to: Methods in Molecular Biology
Publication Type: Book / Chapter
Publication Acceptance Date: 9/14/2020
Publication Date: 11/30/2021
Citation: Krueger, R., Vidalakis, G. 2021. Study and detection of citrus viroids in woody hosts. In: Rao, A.L.N., Lavagi-Craddock, I., Vidalakis, G., editors. Viroids. Methods in Molecular Biology, Volume 2316. New York, NY: Humana Press. p. 3-21. https://doi.org/10.1007/978-1-0716-1464-8_1.
Technical Abstract: Biological indexing is based upon the ability of certain plants, referred to as indicator plants or indicators, to produce specific symptoms when inoculated with a pathogen using mechanical means or grafting. In the case of citrus viroids, clonal indicators are grafted on to vigorous rootstocks such as rough lemon. The ‘A-861-S-1’ citron clonal indicator can detect all citrus viroids except CVd-IIb and CVd-IIc, for which the clonal indicator ‘Parson’s special # 9’ mandarin is used. Inoculation techniques and symptom expression is described in detail. Other supporting elements, such as greenhouse conditions and propagation techniques, are also presented.