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ARS Home » Pacific West Area » Riverside, California » National Clonal Germplasm Repository for Citrus » Research » Publications at this Location » Publication #375118

Research Project: Conservation, Management and Distribution of Citrus and Date Genetic Resources and Associated Information

Location: National Clonal Germplasm Repository for Citrus

Title: Study and detection of citrus viroids in woody hosts

item Krueger, Robert
item VIDALAKIS, GEORGIOS - University Of California

Submitted to: Methods in Molecular Biology
Publication Type: Book / Chapter
Publication Acceptance Date: 9/14/2020
Publication Date: 11/30/2021
Citation: Krueger, R., Vidalakis, G. 2021. Study and detection of citrus viroids in woody hosts. In: Rao, A.L.N., Lavagi-Craddock, I., Vidalakis, G., editors. Viroids. Methods in Molecular Biology, Volume 2316. New York, NY: Humana Press. p. 3-21.

Interpretive Summary:

Technical Abstract: Biological indexing is based upon the ability of certain plants, referred to as indicator plants or indicators, to produce specific symptoms when inoculated with a pathogen using mechanical means or grafting. In the case of citrus viroids, clonal indicators are grafted on to vigorous rootstocks such as rough lemon. The ‘A-861-S-1’ citron clonal indicator can detect all citrus viroids except CVd-IIb and CVd-IIc, for which the clonal indicator ‘Parson’s special # 9’ mandarin is used. Inoculation techniques and symptom expression is described in detail. Other supporting elements, such as greenhouse conditions and propagation techniques, are also presented.