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ARS Home » Southeast Area » Mayaguez, Puerto Rico » Tropical Crops and Germplasm Research » Research » Publications at this Location » Publication #374912

Research Project: Genetic Improvement of Stress Tolerance in Common Bean through Genetic Diversity and Accelerated Phenotyping

Location: Tropical Crops and Germplasm Research

Title: QTL for resistance to angular leaf spot and rust in Tanzania vs South Africa for the Andean panel & Rojo/Cal 143 RIL population

Author
item Miklas, Phillip - Phil
item CHILAGANE, LUSEKO - SOKOINE UNIVERSITY OF AGRICULTURE
item FOURIE, DEIDRE - AGRICULTURAL RESEARCH COUNCIL OF SOUTH AFRICA
item NCHIMBI, SUSAN - SOKOINE UNIVERSITY OF AGRICULTURE
item SOLER-GARZON, ALVARO - WASHINGTON STATE UNIVERSITY
item HART, JOHN - EARTHWORK SEEDS INC.
item MCCLEAN, PHILLIP - NORTH DAKOTA STATE UNIVERSITY
item Pastor Corrales, Marcial - Talo
item Song, Qijian
item Porch, Timothy - Tim

Submitted to: Bean Improvement Cooperative Annual Report
Publication Type: Research Notes
Publication Acceptance Date: 5/18/2020
Publication Date: 5/20/2020
Citation: Miklas, P.N., Chilagane, L., Fourie, D., Nchimbi, S., Soler-Garzon, A., Hart, J., Mcclean, P., Pastor Corrales, M.A., Song, Q., Porch, T.G. 2020. QTL for resistance to angular leaf spot and rust in Tanzania vs South Africa for the Andean panel & Rojo/Cal 143 RIL population. Bean Improvement Cooperative Annual Report. 63: 83-84.

Interpretive Summary: Angular leaf spot (ALS) and bean rust are common bean diseases of global importance. We sought to identify resistance genes in the Andean gene pool effective against ALS and rust in Tanzania and South Africa. The Andean Diversity Panel (ADP) was grown in Cedara, South Africa and in Arusha and Mbeya, Tanzania. The 147 RILs from Rojo (Andean)/CAL 143 (Andean) population (RC pop) were similarly tested. Diseases occurred naturally and were rated from 1=no disease symptoms to 9 = completely susceptible during mid pod filling growth stages. The ADP was genotyped with 9,639 (ARS) and 124,264 (NDSU) SNPs (after filtering) obtained by GBS from the different labs. The RC RILs were genotyped with SNPs from the BARC 6K and 12K Illumina Assays, and by GBS. Following genetic analysis using GWAS and QTL analyses, different QTL for ALS resistance were detected in South Africa vs. Tanzania, while there were common, Pv01 and Pv04, and uncommon QTL detected for rust resistance across locations.

Technical Abstract: Angular leaf spot (ALS) caused by Pseudocercospero griseola and bean rust caused by Uromyces appendiculatus are common bean diseases of global importance. We sought to identify resistance genes in the Andean gene pool effective against ALS and rust in Tanzania and South Africa. The Andean Diversity Panel (ADP) was grown in Cedara, South Africa and in Arusha and Mbeya, Tanzania. The 147 RILs from Rojo (Andean)/CAL 143 (Andean) (RC pop) were similarly tested. Diseases occurred naturally and were rated from 1=no disease symptoms to 9 = completely susceptible during mid pod filling growth stages. The ADP was genotyped with 9,639 (ARS) and 124,264 (NDSU) SNPs (after filtering) obtained by GBS from the different labs. The RC RILs were genotyped with SNPs from the BARC 6K and 12K Illumina Assays, and by GBS. FarmCPU and mrMLM models were used for GWAS of the ADP, and MapDisto and rQTL were used to generate a linkage map and conduct QTL analysis for the RC population. Different QTL for ALS resistance were detected in South Africa vs. Tanzania, while there were common, Pv01 and Pv04, and uncommon QTL detected for rust resistance across locations.