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ARS Home » Southeast Area » Canal Point, Florida » Sugarcane Field Station » Research » Publications at this Location » Publication #373982

Research Project: Development of High-Yielding, Stress Tolerant Sugarcane Cultivars Using Agronomic, Genetic, and Molecular Approaches

Location: Sugarcane Field Station

Title: Comparative proteomic and transcriptomic reveals that crucial metabolic pathways in response to sugarcane ratoon stunting disease

Author
item ZHU, KAI - GUANGXI UNIVERSITY
item Zhao, Duli
item YANG, LITAO - GUANGXI UNIVERSITY
item LI, YANGRUI - GUANGXI UNIVERSITY

Submitted to: American Society of Sugar Cane Technologists
Publication Type: Abstract Only
Publication Acceptance Date: 5/20/2020
Publication Date: 7/15/2020
Citation: Zhu, K., Zhao, D., Yang, L., Li, Y. 2020. Comparative proteomic and transcriptomic reveals that crucial metabolic pathways in response to sugarcane ratoon stunting disease. American Society of Sugar Cane Technologists. 40:24-25.

Interpretive Summary: N/A

Technical Abstract: Sugarcane ratoon stunting disease (RSD), caused by Leifsonia xyli subsp. xyli (Lxx), is one of the most serious bacteria diseases to affect sugarcane production. The molecular basis of sugarcane response to Lxx infection at the proteome level was incomplete and combining proteomic and transcriptional analyses have not yet been conducted. In this study, RNA-Seq analyses of transcriptomes of healthy and Lxx-infected sugarcane (Badila) stalks and leaves were studied, the differentially expressed proteins were also analyzed by employing a data-independent acquisition (DIA)-mass spectrometry (MS). The proteome and transcriptome quantitative data were validated by multiple reaction monitoring (MRM) and qRT-PCR, respectively. We identified 98 and 407 differentially expressed proteins in sugarcane leaves and stalks at 90 days after inoculation with Lxx. The integrative analysis showed that the correlations between the differentially expressed proteins and the corresponding genes that were obtained in transcriptome study were poor with r = 0.1545 in leaves and 0.3076 in stalks. Therefore, this study revealed a post-transcriptional event during leaf-Lxx and stalk-Lxx compatible interaction and sugarcane stalks were more sensitively response to Lxx than leaves. We also found that some metabolic pathways, such as photosynthesis, phytohormone biosynthesis, phytohormone action-mediated regulation, plant-pathogen interactions, and/or protein phosphorylation/dephosphorylation, implicated an active defense response to RSD infection.