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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #369051

Research Project: Intervention Strategies to Control Endemic and New and Emerging Viral Diseases of Swine

Location: Virus and Prion Research

Title: Detection of pseudorabies virus (PRV) antibody in serum and oral fluid specimens from inoculated and/or vaccinated pigs using a whole virus indirect IgG ELISA

item CHENG, TIN-YU - Iowa State University
item Buckley, Alexandra
item VAN GEELEN, ALBERT - Animal And Plant Health Inspection Service (APHIS)
item Lager, Kelly
item HENAO-DIAZ, ALEXANDRA - Iowa State University
item POONSUK, KORAKRIT - Iowa State University
item PINEYRO, PABLO - Iowa State University
item WANG, CHONG - Iowa State University
item BAUM, DAVE - Iowa State University
item MAIN, ROGER - Iowa State University
item ZIMMERMAN, JEFF - Iowa State University
item GIMENEZ-LIROLA, LUIS - Iowa State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/21/2019
Publication Date: 11/4/2019
Citation: Cheng, T., Buckley, A.C., Van Geelen, A., Lager, K.M., Henao-Diaz, A., Poonsuk, K., Pineyro, P., Wang, C., Baum, D., Main, R., Zimmerman, J., Gimenez-Lirola, L. 2019. Detection of pseudorabies virus (PRV) antibody in serum and oral fluid specimens from inoculated and/or vaccinated pigs using a whole virus indirect IgG ELISA. Meeting Abstract. Paper No. 45.

Interpretive Summary:

Technical Abstract: OBJECTIVE:Evaluate PRV antibody ontogeny in oral fluid specimens using a commercial PRV indirect ELISA kit (IDEXX Laboratories, Inc., Westbrook, ME) originally designed for the detection of serum antibody. METHODS:Oral fluid and serum samples of known PRV infection were obtained from 12- to 16-week-old pigs in 4 treatment groups (10 pigs per group): negative control (NC), wild-type PRV inoculated (PRV), PRV vaccinated (MLV), and PRV vaccinated and challenged (MLV_PRV) at 21 days post vaccination (dpv) . Depending on the group, serum and oral fluid samples were collected from individual animals over a period of up to 62 dpv. Serum samples were tested as prescribed by the manufacturer; oral fluid samples were tested using a modified protocol. RESULTS:PRV antibody ontogeny in serum samples and oral fluids showed a similar response pattern: PRV antibody was detected in serum by 7 days post-inoculation (dpi) or dpv and in oral fluid by 10 dpi or dpv. A rapid and strong anamnestic response was observed in vaccinated/inoculated pigs. In contrast, no serum or oral fluid antibodies were detected in negative control animals. CONCLUSIONS:PRV-vaccinated/-inoculated pigs generated detectable antibodies in oral fluid over time, i.e., 1) PRV-specific antibodies could be detected in oral fluid specimens and 2) the dynamic pattern of antibodies induced by wild-type PRV was comparable in oral fluid to that in serum. Research in progress will focus on the optimization of the commercial PRV indirect ELISA for oral fluids in terms of diagnostic sensitivity/specificity and repeatability.